Generation of RAG 1-and 2-deficient rabbits by embryo microinjection of TALENs

摘要：

Dear Editor,
The traditional approach for the generation of genetargeted animals is homologous recombination (HR) conducted in embryonic stem (ES) cells followed by chimera technology,such as in mice and rats,or HR in somatic cells combined with nuclear transfer in those animals in which germline-competent ES cells are not available,such as pigs,sheep,goats,and cattle.However,no germline-competent rabbit ES cells are yet available.Furthermore,the efficiency of rabbit cloning is so low that no gene-targeted rabbits have been produced thus far by somatic cell nuclear transfer approaches.Over the past several years,zinc-finger nucleases (ZFNs) technology has been employed to produce gene knockout (KO) animals,such as rats [1],pigs [2],and rabbits [3].

所属期刊栏目：23

资助基金：grants from the Ministry of Science and Technology National Basic Research Program of China973 program2011CB944203;"Strategic Priority Research Program" of the Chinese Academy of SciencesXDA01020107;the National Natural Science Foundation of China31071293;National Science and Technology Major Project2009ZX10004-405;Science and Technology Planning Project of Guangdong Province,China2011A060901019;Innovative Research Team in UniversityIRT1248

在线出版日期：2013-10-16（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：1059-1062