Developmental pruning of early-stage myelin segments during CNS myelination in vivo
Dear Editor,
Axon-wrapping myelin sheath is essential for proper functioning of the nervous system.Myelin is formed from membranous sheets that are elaborated by oligodendrocytes (OLs) in the central nervous system (CNS).Although much is known about the development and migration of OL and its progenitor cell in vitro and in vivo [1-3],how CNS myelin is dynamically formed through OL processes is still unclear [1,3].To examine the process of CNS myelination in vivo,we performed longterm time-lapse confocal imaging of the same OLs in the spinal cord of intact zebrafish larvae during 2-7 days post-fertilization (dpf) with intervals of 2-12 h.Individual OLs were mosaically labeled by membrane-tethered red fluorescent protein (mRFP) under the control of zebrafish Sox10 regulatory DNA [2].We find that,besides extension of some early-stage axon-wrapping myelin segments,28％ segments undergo pruning in an axon diameter-correlated manner during early development.Downregulating neuregulin-ErbB signaling impairs the pruning but not extension.Thus,our study reveals earlystage myelin pruning as a novel process during myelination.
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grants to CH from National Key Basic Research Program2011CB504401;National Natural Science Foundation31130024,31070922;to JLD.from 973 Program2011CBA00400,2012CB945101
2013-08-08（万方平台首次上网日期，不代表论文的发表时间）
共3页
962-964
