Generation of gene-modified mice via Cas9/RNA-mediated gene targeting
Dear Editor,
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) adaptive immune systems are found in bacteria and archaea to protect the hosts against the invasion of viruses and plasmids [1-3].Three types (I-III) of CRISPR systems with different features have been identified.The CRISPR-associated protein Cas9 that belongs to the type II CRISPR/Cas system has attracted much attention due to its potential use in genomic engineering.Cas9 contains one HNH motif and three RuvC-like motifs,homologous to HNH and RuvC endonucleases,respectively (Supplementary information,Figure S1) [4-8].Recent studies showed that Cas9 displayed strong DNA cleavage activity in bacteria and in test tubes.Its nuclease activity is guided by two non-coding RNA elements of the system;one is crRNA (CRISPR RNA) that contains about 20 base pairs (bp) of unique target sequence (called spacer sequence) and the other is tracrRNA (trans-activating crRNA).
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National Basic Research Program of China973 Program,2010CB945101;the National Natural Science Foundation of China31171377
2013-07-01(万方平台首次上网日期,不代表论文的发表时间)
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720-723