Piglets cloned from induced pluripotent stem cells

摘要：

Dear Editor,
Embryonic stem (ES) cells are powerful tools for generating genetically modified animals that can assist in advancing our knowledge of mammalian physiology and disease.Pigs provide outstanding models of human genetic diseases due to the striking similarities to human anatomy,physiology and genetics,but progress with porcine genetic engineering has been hampered by the lack of germline-competent pig ES cells.To overcome this limitation,genetically modified pigs have been produced using genetically modified somatic cells and nuclear transfer (NT).Yet,somatic cells exhibit limited proliferative capacity and have an extremely low frequency of homologous recombination compared to ES cells.Hence,only a few knockout pig models have been reported thus far using standard gene-targeting approaches.

所属期刊栏目：23

资助基金：grants from the Ministry of Science and Technology National Basic Research Program of China 973program2009CB941001,2011CBA01000,2011CB944203,2011 CB965101;the National Science Fund for Distinguished Young Scholars31025016;the National Natural Science Foundation of China31071293;Major Program of Knowledge Innovation Plan,CASKSCX2-YW-R-227;Science and Technology Planning Project of Guangdong Province,China2011A060901019

在线出版日期：2013-03-28（万方平台首次上网日期，不代表论文的发表时间）

页数：共5页

页码：162-166