Promotion of the efficient metabolic maturation of human pluripotent stem cell-derived hepatocytes by correcting specification defects
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Dear Editor,
Promoting the functional maturation of the desired cell types derived from human pluripotent stem cells (hPSCs) remains a major challenge,especially for hepatocytes,as routine access to metabolically functional hepatocytes would enable their use in drug toxicity screening.Although previous attempts to induce hepatic specification from hPSCs yielded cells possessing some hepatic features [1-3],most of these cells showed poor metabolic activities,and the responsible mechanisms are unknown.In this study,we explored the intrinsic defects in hPSCderived immature hepatocytes and tested whether correcting these defects would promote the metabolic maturation of the differentiated cells.
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grants from the National Basic Research Program of China973program, 2012CD966401 and 2009CB522501;Bill & Melinda Gates Foundation Grant1023963 and 37871;the Ministry of Education of China111 project;Beijing Science and Technology PlanZ121100005212001;National Science and Technology Major Project 2012ZX10004-503 to HD