Structural insights into DndE from Escherichia coli B7A involved in DNA phosphorothioation modification

摘要：

Dear Editor,
DNA phosphorothioate (PT) modification,originally developed as an artificial tool to stabilize oligodeoxynucleotides against nuclease degradation [1],was recently found to be incorporated with sulfur into DNA backbone as a novel physiological variation by the five-gene dnd cluster (dndA-dndE) products in a sequence-and stereospecific manner [2].This PT modification causes the DNA degradation (Dnd) phenotype and is widespread and quantized in bacterial genomes,working as a part of a restriction modification system [3-5].This modification can be specifically cleaved in vitro by type Ⅳ restriction endonuclease [6].

所属期刊栏目：22

资助基金：National Basic Research Program of China2009CB918600 and 2011CB966300;National Natural Science Foundation of China20872169,20905074 and 20921091;National New Drug Design Program from Ministry of Health of China2011ZX09506;Pujiang Talents Awards from Science and Technology Commission of Shanghai Municipality08PJl4117

在线出版日期：2013-03-08（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：1203-1206