Essential role of Fbxl14 ubiquitin ligase in regulation of vertebrate axis formation through modulating Mkp3 level

摘要：

Dear Editor,The F-box family of proteins (FBPs),the substrate-recognition components of the Skpl-Cull-F-box-protein (SCF) ubiquitin ligase,are evolutionarily conserved in various species and exhibit key roles in a wide array of biological processes,including tumorigenesis and development ”1-3”.Although approximately 70 FBPs have been identified in humans ”4,5”,only a few of them have been well characterized.Fbxl14 (F-box and leucine-rich repeat protein 14) has been reported to regulate Snail2 protein during neural crest development of Xenopus laevis embryos ”6” and to control Snaill protein stability in mammalian cells ”7”.There are two homologs of Fbxl14 in zebrafish,namely Fbxl14a and Fbxl14b.In this study,we show that Fbxl14a and Fbxl14b are differential regulators of the dorsoventral (DV) patterning of zebrafish embryos and further demonstrate that their roles in axis formation are differentially executed through the modulation of MAP kinase phosphatase-3 (Mkp3) ubiquitination and protein levels.

机标关键词：biological processes、protein stability、mammalian cells、Xenopus laevis、MAP kinase

所属期刊栏目：22

分类号：Q2(细胞生物学)

资助基金：the National Basic Research Program of China 973 Program2011CB966301;2007CB947100;the Shanghai Municipal Commission for Science and Technology074319111;07DZ22919

在线出版日期：2012-10-29（万方平台首次上网日期，不代表论文的发表时间）

页码：936-940