Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle
Dear Editor,Gene targeting is in widespread use as a gold standard for determining the function of genes in mice and human embryonic stem cells ”1”.However,the poor efficiency of this technology has hindered its application to domestic animals,for which embryonic stem cells are not available.Although gene-targeted large domestic animals have been produced successfully by combination of homologous recombination-based targeting strategy and cloning ”2-4”,the efficiency is very low and,more importantly,the disruption of the targeted gene is usually mono-allelic.It thus takes a long time to obtain a null mutant.
BLG、embryonic stem cells、domestic animals、combination、application、technology、strategy、function、human、time、mice、long、use
21
Q2(细胞生物学)
2012-01-18(万方平台首次上网日期,不代表论文的发表时间)
1638-1640