Determining intracellular temperature at single-cell levelby a novel thermocouple method
Dear Editor,Living cells can change their intramembranous temperature during cell activities such as division,gene expression,enzyme reaction,and metabolism ”1,2”.Moreover,under external stimuli,such as drugs or other signals,cells may quickly change their metabolic activities,leading to acute variation of intracellular temperatures from the normal state ”3,4”.However,such temperature change inside cells is usually at a small scale and is of transient nature due to the thermo-influence by the extracellular environment,rendering it rather difficult to measure using the conventional temperature detection methods.Thus,a more precise and faster-response thermometer is needed to measure single-cell temperature changes in real time,which may constitute a new layer of cellular information for studies of cellular signaling,and even clinical diagnosis and therapy.
activities、temperature changes、clinical diagnosis、cells、conventional、information、real time、detection、studies、nature、enzyme、during、small、new
21
R329.2(人体形态学)
2011-12-23（万方平台首次上网日期，不代表论文的发表时间）
1517-1519
