Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells
Dear Editor,Embryonic stem cell (ES cell) lines were first generated by culturing mouse inner cell mass (ICM) on feeder layers in 1981 ”1”.However,in large domestic animals,attempts to establish ES cell lines from ICM of blastocysts or the later epiblast have not been successful.This has hindered the efficient production of genetically modified livestocks by using ES-based approaches.Recently,it was found that ectopic expression of various combinations oftranscriptinn factors is able to reprogram somatic cells to a pluripotent state ”2-5”.These induced pluripotent stem (iPS) cells show similarities to embryo-derived ES cells and can be used to produce viable mice through tetraploid complementation ”6,7”.So far,iPS cells of several mammalian species have been successfully generated ”2,3,8-12”.In this letter,we report the first establishment of bovine iPS cells using defined transcription factors and a modified culture medium.
fibroblast cells、transcription factors、domestic animals、inner cell mass、somatic cells、cell lines、stem cell、iPS、ES cells、species、defined、culture、ICM、feeder、found、mice、far
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R329.2(人体形态学)
2011-12-23(万方平台首次上网日期,不代表论文的发表时间)
1509-1512