Human peripheral blood-born hematosphere as a niche for hematopoietic stem cell expansion

摘要：

@@ Dear Editor,Transplantation of autologous hematopoietic stem/progenitor cells (HSPCs) derived from the adult peripheral blood has been widely used in the treatment of various hematological diseases ”1”.However,the small number of circulating HSPC is the major limitation and necessitates additional interventions such as G-CSF mobilization and leukapheresis.There have been several attempts to overcome the limitation with ex vivo expansion of HSPC.These strategies are largely based on supplementation of one or more ”stem cell niche components”such as supporting-cells,growth factors,extracellular matrix (ECM) or physicochemical microenvironment in the bone marrow ”2”.Spheroid culture methods of stem ceils from different tissues have been successfully used for expansion of cardiac and neural stem cells.These spheres sensitize target stem cells to growth factors and provide sufficient cell-to-cell and cell-to-matrix contacts,mimicking the in vivo stem cell niche ”3,4”.Here we asked whether spheroid culture of blood mononuclear cells (MNCs) would potentiate the expansion of circulating blood HSPC.

机标关键词：cell expansion、stem cells、growth factors、autologous hematopoietic、hematological diseases、mononuclear cells、ex vivo expansion、peripheral blood、culture、matrix、bone marrow、strategies、methods of、treatment、different、used in、neural、would、small、major

所属期刊栏目：21

分类号：Q343(遗传学分支学科)

资助基金：the National Research Foundation funded by the Korea Government MEST2010-0020257;the Innovative Research Institute for Cell Therapy,Republic of Korea

在线出版日期：2011-09-08（万方平台首次上网日期，不代表论文的发表时间）

页码：987-990