Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning

摘要：

@@ Dear Editor,Gene targeting in mouse embryonic stem (ES) cells has revolutionized the field of mouse genetics and allowed for the analysis of diverse aspects of gene function in vivo.For more than two decades,researchers have been actively searching for ES cells from large animals such as pigs and cattle.Unfortunately,to date,no ES cell lines from large animals have passed the crucial test of germ line contribution.The sole method of gene targeting to date in these species remains somatic cell nuclear transfer (SCNT) combined with DNA homologous recombination (HR).Due to the limited proliferation competency and extremely low frequency of HR in somatic cells (less than 10-6),this process is highly inefficient and only a few successful examples have been achieved,even though enrichment strategies such as positivenegative marker selection,promoter-trap and adenoassociated viral delivery were previously used ”1-3”.The low efficiency of gene targeting in cultured somatic cells is the main barrier for gene targeting in large animals.Recently,zinc-finger nuclease (ZFN) technology has emerged as a powerful tool for genome editing.The success of ZFN technology for gene targeting in fruit flies,zebra fish,rodents as well as human cell lines encouraged us to establish a high-efficiency gene-targeting platform in large animals such as pigs ”4-8”.

机标关键词：PPAR、nuclear transfer、gene targeting、somatic cells、homologous recombination、technology、cell lines、low frequency、successful、strategies、method of、function、ES cells、analysis、species、remains、limited、aspects、zebra、human

所属期刊栏目：21

分类号：Q78(基因工程（遗传工程）)

资助基金：the National High Technology Research and Development Program of China2006AA02A103;the National Basic Research Program of China 973 Program2009CB941001;2011CB944204;the Hundred Talents Project of Chinese Academy of SciencesA0858;the Natural Science Foundation of Guangdong Province,China9251066302000001;the Knowledge Innovation Program of the Chinese Academy of SciencesKSCX2-YW-R-227;the National Natural Science Foundation of China31071172;the National Institutes of HealthHL68878;HL89544;established investigator of the American Heart Association0840025N

在线出版日期：2011-09-08（万方平台首次上网日期，不代表论文的发表时间）

页码：979-982