Targeted conditional gene knockout in human embryonic stem cells
@@ Dear Editor, We report our application of a promoterless knockout(KO) strategy combined with Flpase- and Cre-mediated recombination to efficiently ablate gene function in hu-man embryonic stem (hES) cells. Using this strategy, we modified and deleted both alleles of BAF250a (ARID1A), a signature component of the ATP-dependent SWI/ SNF chromatin remodeling complex BAF. This strategy should facilitate the functional studies of genes essential for hES cell self-renewal and differentiation, and be in-strumental for human therapeutic applications.
embryonic stem cells、human、strategy、application、essential、function、studies、complex、hES
20
Q2(细胞生物学)
2010-05-04(万方平台首次上网日期,不代表论文的发表时间)
共4页
379-382