Enhancement of RNAi by a small molecule antibiotic enoxacin

摘要：

@@ Dear Editor,RNAi has become a mainstream molecular tool for assessing the functions of genes in mammalian cells ”1”.Large-scale RNA interference-based analyses are often complicated by false positive and negative hits due to off-target effects ”2” and interferon response ”3”,which can be attributed at least in part to the use of high concentrations of siRNA.Lowering the amounts of siRNAs and shRNAs can effectively and expediently mitigate the off-target effect and interferon response ”4”.

机标关键词：small molecule、mammalian cells、molecular、positive、negative、RNAi、hits、high、use

所属期刊栏目：18

分类号：Q2(细胞生物学)

资助基金：the National Key Project for Basic Research of China2003CB114403;国家自然科学基金20272029;20572053;20421202;20432010;教育部资助项目104189;Nankai University

在线出版日期：2009-04-29（万方平台首次上网日期，不代表论文的发表时间）

页数：共3页

页码：1077-1079