Targeted gene disruption by use of a group Ⅱ intron (tarqetron)vector in Clostridium acetobutylicum
@@ Dear Editor:Clostridium acetobutylicum,a gram-positive,anaerobic,spore-forming bacterium,is capable of using a wide variety of carbon sources to produce acetone,butanol and ethanol.To improve solvent productivity of C.acetobutylicum.metabolic engineering is considered as a useful tool in developing strains with industrially desirable characteristics.However,to date,there are few useful methods for genetic manipulation of C.acetobutylicum,especially for gene disruption.To our knowledge,two types of vectors,including non-replicative and replicative integrative plasmids,have been developed for gene-inactivation in C.acetobutylicum.By using non-replicative integrative plasmids,buk and solR genes of C. acetobutylicum were inactivated”1,2”.
genetic manipulation、methods for
17
Q2(细胞生物学)
2008-05-28(万方平台首次上网日期,不代表论文的发表时间)
共3页
963-965