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10.1038/cr.2007.91

Targeted gene disruption by use of a group Ⅱ intron (tarqetron)vector in Clostridium acetobutylicum

引用
@@ Dear Editor:Clostridium acetobutylicum,a gram-positive,anaerobic,spore-forming bacterium,is capable of using a wide variety of carbon sources to produce acetone,butanol and ethanol.To improve solvent productivity of C.acetobutylicum.metabolic engineering is considered as a useful tool in developing strains with industrially desirable characteristics.However,to date,there are few useful methods for genetic manipulation of C.acetobutylicum,especially for gene disruption.To our knowledge,two types of vectors,including non-replicative and replicative integrative plasmids,have been developed for gene-inactivation in C.acetobutylicum.By using non-replicative integrative plasmids,buk and solR genes of C. acetobutylicum were inactivated”1,2”.

genetic manipulation、methods for

17

Q2(细胞生物学)

2008-05-28(万方平台首次上网日期,不代表论文的发表时间)

共3页

963-965

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细胞研究(英文版)

1001-0602(Print);1748-7838(Onl

31-1568

17

2007,17(11)

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