Photoconversion of Lysotracker Red to a green fluorescent molecule

摘要：

@@ Dear Editor:Lysotracker Red DND-99 (Invitrogen-Molecular Probes)is a fluorophore in the form of a conjugated multi-pyrrole ring structure containing a weakly basic amine that selectively accumulates in acidic compartments and exhibits red fluorescence(excitation:577 nm,emission:590 nm)(Figure 1A).It iS structurally related to Lvsotracker Green (Figure 1B)but has an additional pyrrole ring in conjugation with the primary structure,which produces a longer wavelength emission.Lysotracker Red is commonly used in multicolor imaging studies as a lysosomal marker to determine intracellular localization of a protein of interest by fluorescence and confocal microscopy”1-5” and is recommended by the manufacturer for this application.

机标关键词：intracellular localization、confocal microscopy、primary structure、used in、form of

所属期刊栏目：17

分类号：Q2(细胞生物学)

在线出版日期：2008-05-28（万方平台首次上网日期，不代表论文的发表时间）

页数：共3页

页码：956-958