A RAPID PCR-QUALITY DNA EXTRACTION METHOD IN FISH
PCR has been a general preferred method for biological research in fish,and previous research have enabled us to extract and purify PCR-quality DNA templates in laboratories ”1-4”.The same problem among these procedures is waiting for tissue digesting for a long time.The overabundance time spent on PCR-quality DNA extraction restricts the efficiency of PCR assay,especially in large-scale PCR amplification,such as SSR-based genetic-mapping construction ”5,6”,identification of germ plasm resource”7,8” and evolution research ”9,10”,etc.In this study,a stable and rapid PCR-quality DNA extraction method was explored,using a modified alkaline lysis protocol.Extracting DNA for PCR only takes approximately 25 minutes.This stable and rapid DNA extraction method could save much laboratory time and promotes.
FISH、EXTRACTION METHOD、DNA extraction、extraction method、research、PCR、time、germ plasm、especially、study、long、fish
36
Q3-3
Special Fund for Agro-scientific Research in the Public Interest:200903045;Natural Science Foundation of China:31101894
2012-06-27(万方平台首次上网日期,不代表论文的发表时间)
共3页
365-367