蛋白酶活性检定及其在特定基质1D与2D凝胶中的特性:一种生物样品中分离、鉴定新的活性型蛋白酶的方法

摘要：

@@ The emergence of bacterial pathogen resistance to common antibiotics strongly supports the necessity to develop alternative mechanisms for combating drug-resistant forms of these infective organisms. Currently, few pharmaceutical companies have attempted to investigate the possibility of interrupting metabolic pathways other than those that are known to be involved in cell wall biosynthesis. Bacterial proteases have been showed to play an important role during infection and their inhibitors can retard the growth, proliferation and invasion of bacterial pathogens. To separate and identify these proteases, we have developed a specific, sensitive assay in SDS-polyacrylamide gels after 2D electrophoresis. This method allows simultaneous determination of protease cleavage specificity, molecular weight, isoelectric point, and if necessary, amino acid sequencing.

机标关键词：蛋白酶、酶活性、检定、基质、凝胶、特性、生物样品、鉴定、方法、activity assay、determination of、the growth、cell wall

所属期刊栏目：25

分类号：Q5(生物化学)

在线出版日期：2004-03-19（万方平台首次上网日期，不代表论文的发表时间）

页数：共2页

页码：127-128