10.3969/j.issn.1671-1521.2013.04.003
UPLC法测定西洋参中7种人参皂苷含量
建立同时测定西洋参中7种人参皂苷含量的快速检测方法.方法:采用超高效液相色谱法,ACQUITY UPLC BEH C18色谱柱(2.1 ×50mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速:0.5ml/min,柱温35℃,进样体积2μL,检测波长203 nm.结果:人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd存在良好线性关系,线性范围分别为0.0067~0.334mg/ml,0.0151~0.756 mg/ml,0.0130~0.649 mg/ml,0.0043~0.217 mg/ml,0.0044~0.221mg/ml,0.0085~0.424 mg/ml,0.0086~0.43 mg/ml.结论:该方法准确、重现性好,可用于西洋参中人参皂苷含量测定.
超高效液相色谱(UPLC)、西洋参、人参皂苷
25
S56;R28
国家科技支撑计划项目,项目2011BA103B0106
2014-02-28(万方平台首次上网日期,不代表论文的发表时间)
共3页
9-11