人尿激酶原变体(K151E,R154G)在家蚕中的高效表达

摘要：

@@ Thesilkwormexpressionsystemisawell-knowneconomicalwayofoverproducingrecom-binantprotein.Inthisletter,theCdnaofamutantofsinglechainurokinase-typeplasminogenactivator(rscu-PA)gene,mscu-PA(K151E,R154G),wasreconstructedtoincludethenaturalurokinase(u-PAorUK)signalpeptidesequences,whichwasobtainedbyPCRtechnique.Inordertointroducethewholemscu-PAgene(includlngsignalpeptidesequence)intoBmNPVgenomeandputitunderthecontrolofthepolyhedrin(ph)promoter,aBmNPV-drivedtrans-fervectorPbe284wasusedaswellasPvl1392,atransfervectorderivedfromAcNPV.BasedonthesequencehomologyofphpromoteranditsflankingregioninBmNPVandAcNPVgenome,the5'nonencodingsequence(includingpromoter)ofthephgenefromPvl1392re-placedthatinwidetypeBmNPVDNAviainvivohomologousrecombination.Thetwopoly-hedrin-negativerecombinant(re-)viruseswereisolatedandidentifiedbydothybridizationusingalabeledscu-PACdnaprobe,andweredesignedBmNPV-mscu-PA-B(byPbe284)andBmN-PV-mscu-PA-V(byPvl1392)respectively.

关键词：尿激酶原变体、杆状病毒转移载体、BmNPV重组病毒、家蚕细胞及幼虫

所属期刊栏目：36

分类号：Q784(基因工程（遗传工程）)

资助基金：The China National Ptogram of Hingh Technology Developraent863-102-08-02-01

在线出版日期：2008-05-12（万方平台首次上网日期，不代表论文的发表时间）

页数：共4页

页码：235-238