ImmunogenicCharacteristicsofRSVF2ProteinExpressedinE.coliRosetta(DE3)

摘要：

Respiratorysyncytialvirus(RSV)isamajorcauseoflowerrespiratorytractinfectioninchildrenyoungerthan5yearsold.However,asafeandeffectivevaccinehasnotbeendevelopedsofar,thereforeitisnecessarytoemploydifferentstrategiestoacceleratethedevelopmentofpreventativevaccines.Inthisstudy,thegenederivedfromRSVfusion(F)proteinF2subunitisexpressedinE.coliRosetta(DE3)usingrecombinantglutathioneStransferase(GST)taggedprotein(GSTF2)ofvector.ExpressionofrecombinantproteinisidentifiedbySDSPAGEandWesternBlot.KunmingmiceareimmunizedwithpurifiedF2protein.SeraarecollectedafterthreetimesboosttoassesstheimmuneresponsebyELISA.MiceimmunizedwithrecombinantproteinyieldhightitersofIgG(p<0.05),andtheratioofIgG1/IgG2aisalsosignificantlyhigherthanthatofcontrol.However,thereisnosignificantchangeinsecretaryIgA(sIgA)intherespiratorytractmucosallavagefluidofmice(p>0.01).ResultsshowthattheF2proteinisapotentialvaccinecandidateforRSV,whichinducesTh2typeimmuneresponse.

关键词：respiratorysyncytialvirus、immunogeniccharacteristic、F2protein

分类号：R373.1(医学微生物学（病原细菌学、病原微生物学）)

在线出版日期：2012-08-29（万方平台首次上网日期，不代表论文的发表时间）

页数：共7页

页码：54-60