The Simultaneous Coupling of Transcription and Splicing in Plants
Gene expression involves a three-step process including transcribing precursor messenger RNAs (pre-mRNAs) from DNA templates by RNA polymerase Ⅱ (Pol Ⅱ),processing pre-mRNAs into mature mRNAs,and translating mature mRNAs into proteins (Bentley,2014).The maturation of pre-mRNAs entails 5'end capping,pre-mRNA splicing,3'end processing,and RNA modification (Bentley,2014).Pre-mRNA splicing is a two-step transesterification reaction,catalyzed by the spliceosome in recognizing various sequence motifs in pre-mRNAs including 5'and 3'splice sites,branch points,polypyrimidine tracts,and other splicing regulatory elements (Herzel et al.,2017).For a long time,transcription and splicing steps were considered independent since both reactions could be reconstituted in vitro,respectively.However,new imaging and next-generation sequencing methods have revealed that both transcription and splicing occur simultaneously rather than sequentially in vivo,whereas previous studies in yeast and animals suggested that pre-mRNA splicing is mainly co-transcriptional instead of post-transcriptional (Bentley,2014).
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This work is supported by the Biotechnology and Biological Sciences Research Council [BB/L025000/1] and a European Commission Horizon 2020 European Research Council Starting Grant [680324
2020-05-07（万方平台首次上网日期，不代表论文的发表时间）
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