Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs
Dear Editor,
In the past few years,the use of sequence-specific nucleases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits.These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining (NHEJ) or homologous recombination (HR) pathways.NHEJ is often imprecise and can introduce mutations at target sites resulting in the loss of gene function.In contrast,HR uses a homologous DNA template for repair and can be employed to create site-specific sequence modifications or targeted insertions (Moynahan and Jasin,2010).
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The work was supported by the National Natural Science Foundation of ChinaGrant .201263,383601,31200273,and 31271420;the National Science Foundation Grant No.DBI-0923827.No conflict of interest declared
2013-10-21（万方平台首次上网日期，不代表论文的发表时间）
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1365-1368
