Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system
Dear Editor,
The CRISPR system has been widely used for genome manipulation in various cells,tissues and whole organisms.Although an increasing variety of inducible CRISPR systems have been exploited for a variety of applications,such as chemical switch (Zetsche et al.,2015),photo switch(Shao et al.,2018) and solution ligand switch (Baeumler et al.,2017;Kipniss et al.,2017;Schwarz et al.,2017) systems,a cell-cell interaction inducible system is absent.The synthetic Notch (synNotch) receptor is a recently developed cell-cell contact sensing platform,which contains a customized extracellular sensor module,a transmembrane core domain of native Notch,and a customized intracellular responder module (Morsut et al.,2016).Because of its extraordinary flexibility in terms of customizable sensing/response behaviors,the synNotch receptor serves as a powerful tool for cell engineering (Roybal et al.,2016a;Roybal et al.,2016b;He et al.,2017).In the current study,we combined the synNotch receptor with the CRISPR/Cas9 system to develop a cell-cell interaction inducible gene regulated tool.
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We are grateful to all members of the Lin and Rong labs for helpful comments and discussions on the manuscript;This work was supported by the National Natural Science Foundation of China ;National Science and Technology Major Project ;Frontier Research Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory ;the Program of Department of Science and Technology of Guangdong Province ;the Natural Science Foundation of Guangdong Province
2020-06-02（万方平台首次上网日期，不代表论文的发表时间）
共5页
299-303
