Multiple sgRNAs facilitate base editing-mediated i-stop to induce complete and precise gene disruption

摘要：

Dear Editor,Gene editing is a process to introduce desired changes into targeted loci of genomic DNA.Recently,type Ⅱ clustered regularly interspaced short palindromic repeats-associated Cas9 endonuclease (CRISPR/Cas9) system has been demonstrated as a versatile tool for engineering eukaryote genome (Hsu et al.,2014),such as in mice (Zuo et al.,2017).CRISPR/Cas9-mediated genome editing is achieved by the error-prone DNA repair of non-homologous end joining (NHEJ) after double strand DNA cleavage.However,the editing results are unreliable due to uncontrolled random indels.Moreover,it was also occasionally reported that Cas9 may induce troublesome off-target effects (Hsu et al.,2013;Pattanayak et al.,2013;Cho et al.,2014).

机标关键词：DNA cleavage、genomic DNA、DNA repair

所属期刊栏目：10

分类号：O6 ;X17

资助基金：The authors declare no Competing Financial or Non-Financial Interests.This work was supported by National Center for International Research2017B01012;National Natural Science Foundation of ChinaGrant .31771299,81600380 and 31600958;Natural Science Foundation of Jiangsu ProvinceBK20160313,BK20160317

在线出版日期：2019-12-30（万方平台首次上网日期，不代表论文的发表时间）

页数：共8页

页码：832-839