Terminal transfer amplification and sequencing for high-efficiency and low-bias copy number profiling of fragmented DNA samples
Dear Editor,Since its invention,next generation sequencing (NGS) has greatly facilitated biomedical research and clinical diagnosis (Sikkema-Raddatz et al.,2013).Continuous dropping of the cost further accelerated the adaptation of sequencing as a standard analytical tool,from identification of drug candidates (Walker et al.,2015) to deciphering the complex biological systems (McConnell et al.,2013).However,progress in sample preparation technology has not been able to catch the speed of sequencing method evolution.For most NGS platforms,samples with DNA fragments to be sequenced need to be firstly converted into a”library”in which each molecule can be further amplified into clones and then be sequenced.Library preparation is a critical step that generates short DNA fragments with certain adapters,and sometimes with barcodes,at both ends.While library construction from bulk genomic DNA samples is a routine procedure,traditional protocols become challenging when the starting material is limited.Materials from many research and diagnostic fields such as archaeology and preimplantation genetic diagnosis (PGD) (Treff et al.,2013) require DNA amplification before library construction.
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We thank Dr.Xiannian Zhang and Dr.Yusi Fu for technical guidance and assistance,and Biodynamic Optical Imaging Center,Peking University High-throughput Sequencing Center for DNA sequencing assistance.We also thank Dr.Jie Shen for providing cells and Dr.Chen Cao for plant care.The genome sequence des21327808,21525521 to Yanyi Huang and 21675098 to Jianbin Wang;Ministry of Science and Technology of China2015AA0200601 to Yuhong Pang and 2016YFC0900100 to Jianbin Wang and Yuhong Pang;Beijing Advanced Innovation Center for Genomics
2019-05-09（万方平台首次上网日期，不代表论文的发表时间）
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229-233
