Identification of critical base pairs required for CTCF binding in motif M1 and M2
Dear Editor,The ubiquitously expressed CCCTC-binding factor (CTCF),is highly conserved from Drosophila to mammals and plays multiple functions in the genome (Ohlsson et al.,2001).CTCF has been shown to establish chromatin insulation in vertebrate,and it also plays the roles in transcriptional regulation,X-chromosome inactivation,and imprinting of genes (Phillips and Corces,2009).In addition,CTCF plays a pivotal role in genomic organization and loop formation by mediating long-range chromatin interactions between distant loci (Yao et al.,2010;Tang et al.,2015).Several hypotheses have been proposed to explain the diverse functions of CTCF.The popular ‘zinc-finger model’ proposed that the CTCF”s different functions are due to the interplay between the zinc-finger engagement and the underlying sequence differences (Ohlsson et al.,2001).Genome-wide studies have identified that the majority of CTCF binding sites belongs to a set of nonpalindromic CTCF binding sites with a consensus sequence referred to as M1 (Kim et al.,2007;Schmidt et al.,2012).Recently,another binding motif,referred to as M2 and 5-6 bp upstream of M1,has been discovered (Schmidt et al.,2012).Moreover,CTCF zinc fingers (ZFs) 4-8 strongly bind to the M1,while ZFs 7-11 tend to strongly bind to the M2 (Renda et al.,2007;Xiao et al.,2015).In this study,we aim to compare the binding abilities of CTCF to M1 and M2 and determine which bases were requirement for M1 and M2 bind to CTCF.
transcriptional regulation、genomic organization
8
R75;R39
We thank Dr.Gary Felsenfeld at NIH for helpful discussions.This work was supported in part by the National Basic Research Program 973 Program.2015CB964800 and 2016YFA0100400;the National Natural Science Foundation of ChinaGrant 31471210;Guangdong Frontier and Key Technology Innovation Special Grant2016B030229006;Guangdong Natural Science Funds2015A030308003,2015A030310041 and 2016A030313168;Guangzhou Science Technology and Innovation Commission,Dr.Zhibin Wang is supported by NIH/NIEHS R01ES025761 and the One Hundred Talents Project of the Chinese Academy of Sciences to HY
2017-08-18（万方平台首次上网日期，不代表论文的发表时间）
共6页
544-549
