Generation of cell-type-specific gene mutations by expressing the sgRNA of the CRISPR system from the RNA polymerase Ⅱ promoters
Dear Editor,Recently, the CRISPR/Cas9 system is emerging as a powerful tool for genome editing (Chang et al., 2013;Li et al., 2013;Niu et al., 2014;Shen et al., 2013;Wan et al., 2015;Wang et al., 2013) and genetic screening (Konermann et al., 2015), and holds great promise for biomedical applications in disease modeling and gene therapy by in vivo genome editing (Maddalo et al., 2014;Xue et al., 2014).
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The authors thank all members of Laboratory of Embryo Biotechnology in College of life science in Northeast Agricultural University and State Key Laboratory of Reproductive Biology in Institute of Zoology in Chinese Academy of Sciences;this study was supported by the grant from the International S&T Cooperation Programme 2013DFG30680, the grant from the ”Strategic Priority Research Program” of the Chinese Academy of Sciences XDA01010407, and the grant from the National Basic Research Program 973 Program Nos.2011CBA01006 and 2011CB944202.
2015-12-21（万方平台首次上网日期，不代表论文的发表时间）
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