10.3969/j.issn.1004-616X.2001.04.027
27. Molecular spectra of acrylamide-induced mutation at hprt locus inhuman promyelocytic leukemia HL-60 and NB4. cell lines
The genotoxicity of acrylamide was investigated by methods of single cell clone culturing, two-way screening count, multiplex PCR amplification and electrophoresis technique. Acrylamide only showed clear mutagenesis until dose raised 700 mg*L-1 in HL-60 cells. The most frequent spontaneous mutation were point mutation (≥90.0%) and acrylamide-induced mutation mainly included partial deletion and point mutation (respectively 40.0%～66.7%/33.3%～60.0%). Total gene deletion wasn't discovered in both of cells. There were deletion mutation in all exons of hprt gene(except 7/8 exon), and toward the 3' end of the hprt gene. The most frequent acrylamide-induced mutation were point mutation and single exon deletion (93.3%/86.1%). There were not clear difference in both of cells. The results suggest that the spectra of spontaneous and acrylamide-induced mutants were different. and the smaller changes in genetic structure have something to do with mechanism.
gene、hprt、acrylamide、mutagenesis、cell HL-60、cell NB4、multiplex PCR
13
R73(肿瘤学)
国家自然科学基金39970650;Military foundation project98M090;RCP project of City University of Hong Kong9360017
2004-03-26（万方平台首次上网日期，不代表论文的发表时间）
共2页
223-224
