19. The HUman Micro Nucleus project. International Date Base Comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes.Ⅰ. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei
The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus assay in human lymphocytes and participating in the HUMN (HUman MicroNucleus project) international collaborative study are presented. The effects of laboratory protocol, scoring criteria, and host factors on baseline micronucleus(MN) frequency are evaluated, and a reference range of “normal” values against which future studies may be compared is provided. Primary data from historical records were submitted by 25 laboratories distributed in 16 countries. This resulted in a database of nearly 7000 subjects. Potentially significant differences were present in the methods used by participating laboratories, such as in the type of culture medium, the concentration of Cytochalasin-B, the percentage of fetal calf serum, and in the culture method. Differences in criteria for scoring MN were also evident. The overall median MN frequency in non-exposed(i.e., normal) subjects was 6.5‰ and the interquartile range was between 3‰ and 12‰. An increase in MN frequency with age was evident in all but two laboratories. The effect of gender, although not so evident in all databases, was also present, with females having a 19% higher level of MN (95% C.I.:14-24%). Statistical analyses were performed using random-effects models for correlated data. Our best model, which included exposure to genotoxic factors, host factors, methods, and scoring criteria, explained 75% of the total variance, with the largest contribution attributable to laboratory methods.
tokinesis-block micronucleus assay、Peripheral blood lymphocytes、Pooled reanalysis、Biomar-kers
13
R73(肿瘤学)
2004-03-26（万方平台首次上网日期，不代表论文的发表时间）
共3页
217-218
